Experiment: Photosynthesis do not occur without Chlorophyll

Requirements: A Variegated leaf of an Ornamental plant, Alcohol, Iodine solution, and a Beaker.

Procedure: A variegated leaf of the ornamental plant should be collected at noon and its green portion is marked, then it should be boiled in alcohol and then immersed in the iodine solution. Observation: After immersing in the Iodine solution it will be seen that only the green portion of the leaf become blue (deep violet or black).


Conclusion: Due to the presence of chlorophyll only the green portions manufactured carbohydrate by photosynthesis. Having no chlorophyll orange or yellow regions did not manufacture carbohydrate. As there were carbohydrates in green portion, that portion become blue (deep violet or black).