How temperature affect on enzyme catalysed reaction?

• Temperature reaching the enzyme and substrate molecules causes them to raise random movement.
• The larger temperature the extra the molecules move and so the more repeatedly they collide.
• The extra collisions there are the greater the possibility that substrates will fit into an active site, up to an exact temperature.
• At the best temperature of an enzyme, the reaction rate is maximum.
• Temperature also affects the figure of the active site, the active site being the correct shape at the best temperature.
• At temperatures over optimum, the rate of reaction decreases because the active site begins to warp.
• At higher temperature causes the enzyme turn into denatured, i.e. bonding becomes irreversibly changed and the active site is permanently smashed.
• At very high temperatures, the number of collisions is also high, but without active sites, no products can be formed.
• At lower temperatures than the optimum, the rate of the reaction decreases because of reduced enzyme substrate collisions.

Most enzymes have the best temperature of between 30^oC and 40^oC, but there are lots of exceptions. An example of this is shown by various bacteria that live at high temperatures in hot volcanic springs.