How does the enzyme works with substrate?

The process of an enzyme at work:

There are four steps in the working process –

Step-1 :

An enzyme and a substrate are in the same area. The substrate is the biological molecule that the enzymes will work on.

Step-2 :

A special region of the enzyme called the active site has a shape that fits with a specific substrate molecule. An enzyme works by binding one or more specific molecules called substrates at the active site. The enzymes and substrates form an enzyme-substrate complex. Then enzymes shape slightly changes.

Step-3 :

The interaction between the substrate and the enzyme stresses or weakens some of the chemical bonds in the substrates. These stresses encourage a link between the two substrates leading to the formation of a different molecule. As a result of the chemical interactions within the active site, a new product is formed.

Step-4 :

Then the product is released from the active site, the enzyme assumes its original shape and is free to work again. Although this reaction has specifically illustrated the formation of a single product from two substrates molecules, other enzymes catalyze the formation of two products from a single product.

Enzyme action

Figure: shows that the action of the enzyme